Product Description

GPX4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90597-50, BS90597-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P36969(Human) O70325(Mouse) P36970(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat, Zebrafish

Applications: WB, IHC

All Applications: WB:1:500-1:2,000
IHC:1:50-1:200

Background: Glutathione peroxidase (GPx) enzymes are generally selenium-containing tetrameric glycoproteins that help prevent lipid peroxidation of cell membranes. GPx enzymes reduce lipid hydroperoxides to alcohols, and reduce free hydrogen peroxide to water. GPx members are among the few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. There are eight GPx homologs (GPx-1-8). GPx-1, Gpx-2 and Gpx-3 exist as homotetramers. Gpx-4 has a high tendancy to form high molecular weight oligomers.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: GPX4 polyclonal antibody detects endogenous levels of GPX4 protein.

Bioworld Molecular Weight: 22 kDa Clone number: JU11-31

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of GPX4 on different lysates using anti-GPX4 antibody at 1/500 dilution.
Positive control:
Lane 1: Rat liver
Lane 2: Mouse kidney
Lane 3: Human liver
Lane 4: Jurkat, Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GPX4 antibody. Counter stained with hematoxylin.

Alternative Name: PHGPx, GPx-4, GSHPx-4, Glutathione peroxidase 4, GPX 4, GPX-4, GPX4, GPX4, GSHPx-4, MCSP, mitochondrial, PHGPx, Phospholipid hydroperoxidase, Phospholipid hydroperoxide glutathione peroxidase, Phospholipid hydroperoxide glutathione peroxidase mitochondrial, snGPx, snPHGPx, Sperm nucleus glutathione peroxidase,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified