GR Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB9238-50, MB9238-100
Product: Mouse IgG2a, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P04150(Human)
Host: Mouse
Reactivity: Human
Applications: WB, ICC IHC FC
All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: The glucocorticoid receptor (GR) is a ubiquitously expressed transcription factor that mediates the effects of glucocorticoids. The most abundant isoform is GR α. GR induces or represses the expression of genes in response to glucocorticoids, mediating such processes as apoptosis, cell growth and differentiation. A significant class of genes suppressed by GR is controlled by the transcription factor AP-1. GR has also been shown to be the limiting factor in the induction of gene expression by glucocorticoids. It has been revealed that GR forms a complex with HSP 90, rendering the non-ligand bound receptor transcriptionally inactive. More importantly, mutant GRs lacking the signaling domain remain constitutively active.
Purification and Purity: ProG affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: GR monoclonal antibody detects endogenous levels of GR protein.
Bioworld Molecular Weight: 86 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Glucocorticoid Receptor alpha on SiHa (1) and A549 (2) cell lysates using anti-Glucocorticoid Receptor alpha antibody at 1/5,000 dilution., ICC staining Glucocorticoid Receptor alpha (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: GR, NR3C1, GRL, GCCR, GCR, Glucocorticoid receptor alpha isoform, Glucocorticoid receptor, GR, GRL, NR3C1, Nuclear receptor subfamily 3 group C member 1,
Immunogen: Recombinant full length protein corresponding to Human Glucocorticoid Receptor alpha.
Conjugate: Unconjugated
Modification: Unmodified