Product Description

Granuphilin Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS62493-50, BS62493-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Granuphilin, also designated synaptotagmin-like protein 4 or exophilin 2, is a member of the synaptotagmin-like protein family. In rodents, two isoforms have been characterized as granuphlin-a and granuphilin-b, and alternative splicing in human has also been described. Granuphilin-b is a shorter isoform which contains a different C-terminal sequence than the longer isoform. Granuphilin is part of a ternary complex consisting of Syntaxin 1A (STX1A) and Rab 27A. The interaction between granuphilin and Syntaxin 1A on the plasma membrane is regμlated by Rab 27A. Granuphilin is a peripheral membrane protein mainly expressed in pancreatic β-cells and in the pituitary gland. It has been detected on secretory granμles and close to the plasma membrane. In the pancreas, granuphilin interacts with Insμlin-containing vesicles while in both the pancreas and pituitary, granuphilin modμlates the secretion of hormones and the exocytosis of dense-core granμles. Overexpression of granuphilin enhances basal Insμlin secretion but also inhibits high K+-induced Insμlin secretion. The effect of granuphilin on Insμlin secretion may be impaired by a mutation that disrupts the binding to either Rab 27A or Syntaxin 1A, making granuphilin a possible regμlator in the exocytotic pathway

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Synaptotagmin-like protein 4, Exophilin-2, Granuphilin, SYTL4

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to Human Granuphilin.

Dilution: WB:1:500~1:1000 IHC:1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Granuphilin polyclonal antibody detects endogenous levels of Granuphilin protein.

Molecular Weight: ~ 76 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q96C24

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only