GRB2 Polyclonal Antibody-BS90599

GRB2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90599-50, BS90599-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P62993

Host: Rabbit

Reactivity: Human, Mouse, rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500
ICC:1:200
IHC:1:200
FC:1:100-1:200

Background: Grb2 is widely expressed and is essential for multiple cellular functions. Inhibition of Grb2 function impairs developmental processes in various organisms and blocks transformation and proliferation of various cell types, and so it is not surprising that a targeted gene disruption of Grb2 in mouse is lethal at an early embryonic stage. Grb2 is best known for its ability to link the epidermal growth factor receptor tyrosine kinase to the activation of Ras and its downstream kinases, ERK1,2. Grb2 is composed of an SH2 domain flanked on each side by an SH3 domain. Grb2 has two closely related proteins with similar domain organizations, Gads and Grap. Gads and Grap are expressed specifically in hematopoietic cells and function in the coordination of tyrosine kinase mediated signal transduction. The SH2 domain of Grb2 binds to phosphorylated tyrosine-containing peptides on receptors or scaffold proteins with a preference for pY-X-N-X, where X is generally a hydrophobic residue such as valine.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: GRB2 polyclonal antibody detects endogenous levels of GRB2 protein.

Bioworld Molecular Weight: 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of GRB2 on different cell lysates using anti-GRB2 antibody at 1/500 dilution., ICC staining GRB2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: GRB2, Abundant SRC homology, Adapter protein GRB2, ASH, Ash protein, EGFRBP GRB2, Epidermal growth factor receptor binding protein, Epidermal growth factor receptor binding protein GRB2, GRB 2, GRB2 adapter protein, Grb2, GRB2, Grb3 3, Growth factor receptor bound protein 2, Growth factor receptor bound protein 3, Growth factor receptor-bound protein 2, HT027, MST084, MSTP084, NCKAP2, OTTHUMP00000166096, OTTHUMP00000166097, OTTHUMP00000166098, Protein ash, SEM5, SH2/SH3 adapter GRB2,

Immunogen: peptide

Conjugate: Unconjugated

Modification: Unmodified