GRF-1 (phospho Tyr1105) rabbit pAb - ES5669

GRF-1 (phospho Tyr1105) rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES5669-50, ES5669-100

Citations, Manuals and MSDS Available upon request.

Background: The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008],

Alternate Name: ARHGAP35; GRF1; GRLF1; KIAA1722; Rho GTPase-activating protein 35; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A; p190-A

Source: Rabbit

Applications: WB; IHC; IF; ELISA

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Reactivity: Human; Mouse; Rat

Immunogen: The antiserum was produced against synthesized peptide derived from human GRF-1 around the phosphorylation site of Tyr1105. AA range:1071-1120

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 160kD

Human Gene ID: 2909

Human SWISS Prot NO: Q9NRY4

Subcellular Location: Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm. Nucleus. Cell membrane. In response to integrins and SDC4 and upon phosphorylation by PKC, relocalizes from the cytoplasm to regions of plasma membrane ruffling where it colocalizes with polymerized actin.

Research Use Only