Product Description

GRF-1 (phospho-Y1087) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4814-50, BS4814-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: GRF-1 (glucocorticoid receptor DNA-binding factor 1), also known as p190RhoGAP or simply p190, is a transcriptional regμlator which binds to the promoter region of the glucocorticoid receptor gene and represses its expression. By repressing GR expression, GRF-1 acts to down-regμlate Rho signaling, thereby mediating both actin cytoskeletal rearrangements and cell cycle events. Through its GAP domain, GRF-1 is thought to affect cytokinesis by regμlating Rho activity, a regμlation that is controlled by the ubiquination of the GTP binding region and subsequent degradation of GRF-1. Additionally, GRF-1 plays an important role in oligodendrocyte differentiation, a process that is absent in malignant glioma tumors, implicating GRF-1 as a possible tumor suppressor. GRF-1 expression is regμlated by glucocorticoids and the expressed protein exists as two isoforms produced by alternative splicing events.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC

Alternate Names: Rho GTPase-activating protein 35, Glucocorticoid receptor DNA-binding factor 1, Glucocorticoid receptor repression factor 1, GRF-1, Rho GAP p190A, p190-A, ARHGAP35, GRF1, GRLF1, KIAA1722

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human GRF-1 around the phosphorylation site of Tyrosine 1087.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-GRF-1 (Y1087) polyclonal antibody detects endogenous levels of GRF-1 protein only when phosphorylated at Tyr1087.

Molecular Weight: ~ 172 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9NRY4

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only