Product Description

GRF-1 (phospho-Y1105) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4232-50, BS4232-100

Swiss-Prot: Q9NRY4

Host: Rabbit

GRF-1 (phospho-Y1105) Polyclonal Antibody -BS4232

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: GRF-1 (glucocorticoid receptor DNA-binding factor 1), also known as p190RhoGAP or simply p190, is a transcriptional regulator which binds to the promoter region of the glucocorticoid receptor gene and represses its expression. By repressing GR expression, GRF-1 acts to down-regulate Rho signaling, thereby mediating both actin cytoskeletal rearrangements and cell cycle events. Through its GAP domain, GRF-1 is thought to affect cytokinesis by regulating Rho activity; a regulation that is controlled by the ubiquination of the GTP binding region and subsequent degradation of GRF-1. Additionally, GRF-1 plays an important role in oligodendrocyte differentiation, a process that is absent in malignant glioma tumors, implicating GRF-1 as a possible tumor suppressor. GRF-1 expression is regulated by glucocorticoids and the expressed protein exists as two isoforms produced by alternative splicing events.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-GRF-1 (Y1105) polyclonal antibody detects endogenous levels of GRF-1 protein only when phosphorylated at Tyr1105.

Bioworld Molecular Weight: ~ 172 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-GRF-1 (Y1105) pAb at 1:500 dilution
Lane 1: The Heart tissue lysate of Rat (40ug)
Lane 2: K562 whole cell lysate (40ug)
Lane 3: Jurkat whole cell lysate (40ug)
Lane 4: The Blood tissue lysate of Mouse (40ug)
Lane 5: H1792 whole cell lysate (40ug)
Lane 6: HEK293T whole cell lysate (40ug)
Lane 7: SP2/0 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-GRF-1 (Y1105) pAb in paraffin-embedded human brain tissue.

Alternative Name: Rho GTPase-activating protein 35; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A; p190-A; ARHGAP35; GRF1; GRLF1; KIAA1722

Immunogen: Synthetic phosphopeptide derived from human GRF-1 around the phosphorylation site of Tyrosine 1105.

Modification: Phosphorylation

Conjugate: Unconjugated