Product Description

GRK 2 (D635) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1963-50, BS1963-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Heterotrimeric G protein-mediated signal transduction is a dynamically regμlated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonistmediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first are the second messenger-regμlated kinases such as c-AMP dependent protein kinase A and protein kinase C. The second are the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase, GRK 1, two forms of β-adrenergic receptor kinase, GRK 2 (βARK, βARK1) and GRK 3 (βARK2), IT-11 (GRK 4), GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IP

Alternate Names: Beta-adrenergic receptor kinase 1, Beta-ARK-1, β-ARK-1, ARK-β1, G-protein coupled receptor kinase 2, ADRBK1, BARK, BARK1, GRK2, ARK β1, ARKβ1, β-ARK1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Synthetic peptide, corresponding to amino acids 600-650 of Human GRK2.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:10~1:100

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: GRK 2 (D635) polyclonal antibody detects endogenous levels of GRK 2 protein.

Molecular Weight: ~ 80 kDa

Pathway: G Protein-coupled Receptors Signaling to MAPK Erk,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P25098

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only