Product Description

GRK 6 (N22) Polyclonal Antibody

Catalogue Numbers: BS1997-50, BS1997-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P43250

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonistmediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first are the second messenger-regulated kinases such as c-AMP dependent protein kinase A and protein kinase C. The second are the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase, GRK 1; two forms of β-adrenergic receptor kinase, GRK 2 (βARK, βARK1) and GRK 3 (βARK2); IT-11 (GRK 4); GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GRK 6 (N22) polyclonal antibody detects endogenous levels of GRK 6 protein.

Extra Notes: Western blot (WB) analysis of GRK6 (N22) pAb at 1:500 dilution
Lane1:H9C2 whole cell lysate(40ug)
Lane2:CT26 whole cell lysate(40ug)
Lane3:MCF-7 whole cell lysate(40ug)
Lane4:HEK293T whole cell lysate(40ug)
Lane5:HepG2 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 65 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: G protein-coupled receptor kinase 6; G protein-coupled receptor kinase GRK6; GRK6; GPRK6;

Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human GRK 6.

Conjugate: Unconjugated

Modification: Unmodified