GRK1 (A17) Polyclonal Antibody-BS3251

GRK1 (A17) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS3251-50, BS3251-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15835

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

All Applications: IHC: 1:50~1:200

Background: Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonistmediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first class is comprised of the second messengerregulated kinases, such as c-AMP dependent protein kinase A and protein kinase C. The second class includes the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase (GRK 1α and β); two forms of β-adrenergic receptor kinase: GRK 2 (βARK, βARK1) and GRK 3 (βARK2); IT-11 (GRK 4); GRK 5; GRK 6; and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of GRK1 (A17) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 63 kDa

Specificity: GRK1 (A17) polyclonal antibody detects endogenous levels of GRK1 protein.

Alternative Name: Rhodopsin kinase; RK; G protein-coupled receptor kinase 1; RHOK

Immunogen: Synthetic peptide, corresponding to the N-terminal of Human GRK1.

Conjugate: Unconjugated

Modification: Unmodified