GRK2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90601-50, BS90601-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P25098(Human) Q99MK8(Mouse) P26817(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB:1:1,000-1:2,000
IHC:1:50-1:200
Background: Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonist-mediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first class is comprised of the second messenger-regulated kinases, such as c-AMP dependent protein kinase A and protein kinase C. The second class includes the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase (GRK 1), two forms of beta-adrenergic receptor kinase: GRK 2 (betaARK, betaARK1) and GRK 3 (betaARK2), IT-11 (GRK 4), GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: GRK2 polyclonal antibody detects endogenous levels of GRK2 protein.
Bioworld Molecular Weight: 80 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of GRK2 on Hela cell lysates using anti-GRK2 antibody at 1/1,000 dilution., Fig2 Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GRK2 antibody. Counter stained with hematoxylin.
Alternative Name: Beta-ARK-1, ADRBK1, BARK, BARK1, GRK2, ADRBK1, Adrenergic beta receptor kinase 1, ARBK1, BARK, BARK1, Beta adrenergic receptor kinase 1, Beta ARK 1, Beta ARK1, Beta-adrenergic receptor kinase 1, Beta-ARK-1, FLJ16718, G protein coupled receptor kinase 2, G-protein coupled receptor kinase 2, GRK2,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified