Product Description

GRK5 (Q382) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1981-50, BS1981-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P34947

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonist-mediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first class is comprised of the second messenger-regulated kinases, such as c-AMP dependent protein kinase A and protein kinase C. The second class includes the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase (GRK 1), two forms of b-adrenergic receptor kinase: GRK 2 (bARK, bARK1) and GRK 3 (bARK2), IT-11 (GRK 4), GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GRK5 (Q382) polyclonal antibody detects endogenous levels of GRK5 protein.

Bioworld Molecular Weight: ~ 68 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GRK5 (Q382) pAb at 1:1000 dilution
Lane 1: PC3 whole cell lysate (20ug)
Lane 2: HEK293T whole cell lysate (20ug)
Lane 3: H9C2 whole cell lysate (20ug)
Lane 4: MEF whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of GRK5 (Q382) pAb in paraffin-embedded human breast carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of GRK5 (Q382) pAb in paraffin-embedded human lung carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: G protein-coupled receptor kinase 5; G protein-coupled receptor kinase GRK5; GPRK5

Immunogen: Synthetic peptide, corresponding to amino acids 350-400 of Human GRK5.

Conjugate: Unconjugated

Modification: Unmodified