Product Description

GRP1 (K400) Polyclonal Antibody

Catalogue Numbers: BS2942-50, BS2942-100

Sizes: 50µl, 100µl

Alternative Name: Cytohesin-3; ARF nucleotide-binding site opener 3; Protein ARNO3; General receptor of phosphoinositides 1; Grp1; PH; SEC7 and coiled-coil domain-containing protein 3; ARNO3; GRP1; PSCD3; CYTH3

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43739

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: GRP1 (general receptor for phosphoinositides-1) contains a Pleckstrin homology (PH) domain as well as a Sec7 domain. The PH domain has high binding affinity for phosphatidylinositol 3,4,5-trisphosphate Ptdlns(3,4,5)P3), while the Sec7 homology domain is responsible for catalyzing guanine nucleotide exchange of ADP-ribosylation factor (ARF) proteins. GRP1 co-localizes with ARF6 and catalyzes GTP/GDP exchange on ARF6. It is known to interact with Ptdlns(3,4,5)P3 localized to the plasma membrane in vitro and may also be a Ptdlns(3,4,5)P3 receptor. Additionally, GRP1 may regulate protein sorting and membrane trafficking through interaction with the guanosine triphosphate ARF, and may control cell adhesion through interaction with integrins.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GRP1 (K400) polyclonal antibody detects endogenous levels of GRP1 protein.

Bioworld Molecular Weight: ~ 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GRP1 (K400) pAb at 1:2000 dilution
Lane 1: 3T3-L1 whole cell lysate(40ug)
Lane 2: H9C2 whole cell lysate(40ug)
Lane 3: A2780 whole cell lysate(40ug)
Lane 4: H1792 whole cell lysate(40ug)
Lane 5: A375 whole cell lysate(20ug)

Immunogen: Synthetic peptide, corresponding to amino acids 371-420 of Human GRP1.

Conjugate: Unconjugated

Modification: Unmodified