Product Description

GRP78 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66651-50, MB66651-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Secretory and transmembrane proteins are synthesized on polysomes and translocated into the endoplasmic reticμlum (ER). Inside the ER, these proteins are often modified by disμlfide bond formation, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecμlar chaperones including BiP. BiP was identified as an immunoglobμlin heavy chain binding protein in pre-B cells. It was also found to be induced at the protein level by glucose starvation. When protein folding is disturbed inside ER, BiP synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding.

Category: Primary Antibody

Reactivity: Human, Rat

Host: Mouse

Applications: WB, IHC, IF/ICC

Alternate Names: GRP78, 78 kDa glucose-regμlated protein, GRP-78, Endoplasmic reticμlum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobμlin heavy chain-binding protein, BiP

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E.coli.

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/50 - 1/100)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of GRP78 protein.

Molecular Weight: ~ 78 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P11021

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only