GRP78 Monoclonal Antibody-MB66651

GRP78 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66651-50, MB66651-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P11021

Host: Mouse

Reactivity: Human, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/50 - 1/100)

Background: Secretory and transmembrane proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside the ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including BiP. BiP was identified as an immunoglobulin heavy chain binding protein in pre-B cells. It was also found to be induced at the protein level by glucose starvation. When protein folding is disturbed inside ER, BiP synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of GRP78 protein.

Bioworld Molecular Weight: ~ 78 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of GRP78 expression in Hela (A), C6 (B), Lncap (C), MDAMB468 (D) whole cell lysates., Immunohistochemical analysis of GRP78 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.68). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of GRP78 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Alternative Name: GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP

Immunogen: Purified recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified