Product Description

GRP78 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6479-50, BS6479-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P11021

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:2000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70, which are found in both the cytosol and nucleus of mammalian cells, play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GRP78 polyclonal antibody detects endogenous levels of GRP78 protein.

Bioworld Molecular Weight: ~ 78 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using BiP/GRP78 antibody at 1:1000 dilution., Immunohistochemistry of paraffin-embedded rat brain using BiP/GRP78 antibody at dilution of 1:100 (40x lens)., Immunohistochemistry of paraffin-embedded human liver cancer using BiP/GRP78 antibody at dilution of 1:100 (40x lens)., Confocal immunofluorescence analysis of Hela cells using BiP/GRP78 antibody at dilution of 1:200. Blue: DAPI for nuclear staining.

Alternative Name: 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP; HSPA5; GRP78

Immunogen: Recombinant full length Human GRP7.

Conjugate: Unconjugated

Modification: Unmodified