GRP78 Polyclonal Antibody - E-AB-40588

GRP78 Polyclonal Antibody

Sizes: 50μL, 100μL, 200μL

Catalogue Numbers: E-AB-40588-50, E-AB-40588-100, E-AB-40588-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: GRP78

Target Synonym: BiP; GRP-78; HSP70 family protein 5; Heat shock protein family A member 5; Immunoglobulin heavy chain-binding protein; HSPA5

Research Areas: Cancer, Tags and Cell Markers

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: P11021

Background: GRP78 (Glucose-regulated protein 78 kDa; also BiP and HSPA5) is a 72 kDa member of the heat shock protein 70 family of proteins. Intracellularly, GRP78 is an endoplasmic reticulum chaperone that participates in protein folding; extracellularly, it induces IL-10 production from T cells and interacts with Cripto to block TGF-beta signaling. Human GRP78 precursor is 654 amino acids (aa) in length. It contains an 18 aa signal sequence and a 636 aa mature region that shows a hydantoinase A region (aa 145245) and a C-terminal KDEL motif that is present on intracellular GRP78, but absent on secreted GRP78. There is alternative splicing in the signal sequence (aa 110), and multiple single aa substitituion. Over aa 1654, human GRP78 is more than 97% aa identical to mouse and rat GRP78.

Concentration: 1.5mg/mL

Immunogen: Recombinant Human GRP78 protein expressed by E.coli.

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.4

Purification Method: Antigen Affinity Purification

Dilution: WB: 1:500-1000

Calculated MW: 72kDa

Observed MW: 78kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only