GRP94 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90606-50, BS90606-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P14625(Human) P08113(Mouse) Q66HD0(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IHC
All Applications: WB:1:500-1:2,000
ICC:1:100-1:500
IHC:1:100-1:500
Background: Heat shock protein (HSP) molecular chaperones are environmental stress-inducible gene products. The human HSP 90 family includes 17 genes that fall into four classes: HSP90AA, HSP90AB, HSP90B and TRAP. HSP 90 family members guide the normal folding, intracellular disposition and proteolytic turnover of many key regulators of cell growth, differentiation and survival. HSP 90α, also designated HSP90A, HSP 86 and LPS-associated protein 2 (LAP2), is a cytosolic enhancer of inducible nitric-oxide synthase (iNOS), with chaperone activity that is important for the transcriptional activity of p53. HSP 90β, also designated HSP90B, HSP 84 and HSPC2, is a cytosolic protein that participates in signaling pathways with PKC e to protect cells from external damage, particularly in heat shock-mediated events. GRP 94, also known as tumor rejection antigen 1 (TRA1), ECGP and GP96, localizes to the ER, is highly expressed in BGC-823 human gastric carcinoma cells and is upregulated in human endothelial cells in response to hypoxia by HIF-1. TRAP1 (TNF receptor-associated protein 1), also designated HSP 75) is a mitochondrial matrix component that plays a role in the induction of apoptosis in response to reactive oxygen species.
Purification and Purity: Peptide affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: GRP94 polyclonal antibody detects endogenous levels of GRP94 protein.
Bioworld Molecular Weight: 94 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of GRP94 on different cell lysate using anti-GRP94 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: NIH-3T3
Lane 3: PC-12, ICC staining GRP94 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: GRP-94, HSP90B1, GRP94, TRA1, 94 kDa glucose regulated protein, 94 kDa glucose-regulated protein, ECGP, Endoplasmin, Endothelial cell (HBMEC) glycoprotein, ENPL, Glucose regulated protein 94kDa, gp96, gp96 homolog, GRP 94, GRP-94, Heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), member 1, Heat shock protein, 90 kDa, beta, 1, HSP90B1, Stress inducible tumor rejection antigen GP96, TRA1, tumor rejection antigen (gp96) 1, Tumor rejection antigen 1, Tumor rejection antigen gp96, Tumor rejection antigen-1 (gp96),
Immunogen: Peptide.
Conjugate: Unconjugated
Modification: Unmodified