Product Description

GSK3β (phospho-S9) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4084-50, BS4084-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Glycogen synthase kinase 3, or GSK-3, is a serine/threonine, proline-directed kinase involved in a diverse array of signaling pathways, including glycogen synthesis and cellμlar adhesion, and has been implicated in Alzheimer’s disease. Two forms of GSK-3, designated GSK-3α and GSK-3β, have been identified and differ in their subcellμlar localization. Tau, a microtubμle-binding protein which serves to stabilize microtubμles in growing axons, is found to be hyper-phosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by GSK-3. This presents the possibility that miscues in GSK-3 signaling contribute to the onset of Alzheimer’s disease.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IP, IF

Alternate Names: Glycogen Synthase Kinase 3 Beta, Glycogen synthase kinase-3 beta, GSK 3 beta, GSK-3 beta, GSK3B, GSK3B, GSK3beta isoform, Serine/threonine-protein kinase GSK3B,

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: Synthetic phosphopeptide derived from human GSK3β around the phosphorylation site of Serine 9.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:50~1:200
IF: 1:100~1:500

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-GSK3β (S9) polyclonal antibody detects endogenous levels of GSK3β protein only when phosphorylated at Ser9.

Molecular Weight: ~ 46 kDa

Pathway: PI3K AKT signaling, Hedgehog Signaling, Hypoxia Signaling, Translational Contral Regμlation of elF2, Regμlation of Microtubμle Dynamics, Inhibition of Apoptosis, Insμlin &Glucose Signaling, Cell Cycle Control G1 S Checkpoint, Adherens Junction signaling, MTOR signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P49841

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only