Product Description

GTF2F1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS77894-50, BS77894-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P35269

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of various cell lines, using GTF2F1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 74kDa

Alternative Name: BTF4; RAP74; TF2F1; TFIIF; GTF2F1

Immunogen: Recombinant fusion protein of human GTF2F1(NP_002087.2).

Conjugate: Unconjugated

Modification: Unmodified