Product Description

GTF2H4 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71219-50, BS71219-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q92759

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Component of the general transcription and DNA repair factor IIH (TFIIH core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using GTF2H4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

Bioworld Molecular Weight: 52kDa

Specificity: Polyclonal Antibodies

Alternative Name: GTF2H4; P52; TFB2; TFIIH

Immunogen: Recombinant fusion protein of human GTF2H4(NP_001508.1).

Conjugate: Unconjugated

Modification: Unmodified