Guanylyl Cyclase beta 1 (GUCY1B3) Polyclonal Antibody-BS76218

Guanylyl Cyclase beta 1 (GUCY1B3) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76218-50, BS76218-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q02153

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB,1:200 - 1:2000 | IHC,1:50 - 1:200

Background: This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of GTP (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). The encoded protein contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of various cell lines, using Guanylyl Cyclase beta 1 ) antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30S.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 70kDa

Alternative Name: GUCY1B3; GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3; GUCY1B1

Immunogen: Recombinant fusion protein of human Guanylyl Cyclase beta 1 (Guanylyl Cyclase beta 1 (GUCY1B3))(NP_000848.1).

Conjugate: Unconjugated

Modification: Unmodified