Product Description

GUF1 Polyclonal Antibody

Sizes: 20μL, 50μL, 100μL

Catalogue Numbers: BT-AP03863-20, BT-AP03863-50, BT-AP03863-100

Citations, Manuals and MSDS Available upon request.

Background: GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. In western blotting, we got double bands 74 kDa and 70 kDa, due to the N-terminal 1-49 amino acid sequence that could be cut down.

Research Areas: Others

Target Protein: GUF1

Applications: IHC-p, ELISA

Reactivity: Human

Clonality: Polyclonal

Host: Rabbit

Isotype: IgG

Full Product Name: GUF1 Antibody

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

Molecular Weight (Da): 74328

UniProt Accession: Human: Q8N442; Mouse: Q8C3X4

Synonyms: GUF1; Translation factor GUF1; mitochondrial; Elongation factor 4 homolog; EF-4; GTPase GUF1; Ribosomal back-translocase

GeneID: Human: 60558

Research Use Only