Product Description

GW Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67175-50, MB67175-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Mitotic control is important for normal growth, development, and maintenance of all eukaryotic cells. Research studies have demonstrated that inappropriate control of mitosis can lead to genomic instability and cancer. A regμlator of mitosis, Greatwall kinase (Gwl), was first identified in Drosophila melanogaster. Subsequent studies showed that, based on sequence homology and function, microtubμle-associated serine/threonine kinase-like (MASTL) is the human ortholog of Gwl. Regμlation of MASTL/Gwl activation has been shown to be critical for the correct timing of mitosis. Research studies have shown that Gwl is activated by hyperphosphorylation. The phosphorylation of human Gwl at Thr194 and Thr207 by active cyclin B1-cdc2 leads to possible autophosphorylation at Ser875 (Ser883 in Xenopus), which stabilizes the kinase. Activated Gwl phosphorylates α-Endosμlfine (ENSA) and cAMP-regμlated phosphoprotein 19 (ARPP19) at Ser67 and Ser62, respectively. Phosphorylated ENSA and ARPP19 inhibit the activity of the B55 subunit-associated form of protein phosphatase 2A (PP2A-B55), allowing for complete phosphorylation of mitotic substrates by cyclin B1-cdc2 and mitotic entry. When Gwl is inactivated, PP2A-B55 reactivates, which leads to dephosphorylation of cyclin B1-cdc2 and mitotic exit.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB

Alternate Names: GW, GWL, THC2, Serine/threonine-protein kinase greatwall, GW, GWL, hGWL, Microtubμle-associated serine/threonine-protein kinase-like, MAST-L

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human GW. The exact sequence is proprietary.

Dilution: WB (1/1000 - 1/5000)

Specificity: Recognizes endogenous levels of GW protein.

Molecular Weight: ~ 95 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q96GX5

Product: Mouse IgG1 kappa. Supplied in crude ascites with 0.01% sodium azide.

Research Use Only