GZMM Polyclonal Antibody
Catalogue Numbers: BS60988-50, BS60988-100
Sizes: 50µl, 100µl
Alternative Name: Granzyme M (EC:3.4.21.-); Met-1 serine protease; Hu-Met-1; Met-ase; Natural killer cell granular protease; GZMM; MET1
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P51124
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: The granzyme family of proteins belong to the larger peptidase S1 family. Granzyme A and granzyme B are serine proteases that facilitate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Within the granules of activated CTLs, granzyme A and granzyme B are processed and converted to their active forms by the lysosomal cysteine protease cathepsin C. Once cleaved, these active proteases target distinct substrates for proteolysis, thereby mediating apoptosis through two different pathways. Granzyme H localizes to cytoplasmic granules of cytolytic T lymphocytes and is important for target cell lysis in cell-mediated immune responses. Granzyme K is a serine protease localizing to the granules of NK cells and cytotoxic T lymphocytes. Granzyme M (also designated NK cell granular protease, HU-Met-1 or met-1 serine protease) contains one peptidase S1 domain. Granzyme M is a Trypsin-fold serine protease that localizes to granules of large granular lymphocytes (NK cells) and cleaves peptide substrates after leucine, norleucine or methionine. This enzyme may play a role in target cell death induction by cytotoxic lymphocytes.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: GZMM polyclonal antibody detects endogenous levels of GZMM protein.
Bioworld Molecular Weight: ~ 28 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of GZMM polyclonal antibody at 1:500 dilution
Line1:Hela whole cell lysate
Line2:PC12 whole cell lysate
Line3:sp20 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human GZMM.
Conjugate: Unconjugated
Modification: Unmodified