Product Description

HAND1 Polyclonal Antibody

Catalogue Numbers: BS61014-50, BS61014-100

Sizes: 50µl, 100µl

Alternative Name: Heart- and neural crest derivatives-expressed protein 1; Class A basic helix-loop-helix protein 27; bHLHa27; Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1; eHAND; HAND1; BHLHA27; EHAND

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O96004

Host: Rabbit

Reactivity: Human, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: dHAND (for deciduum, heart, autonomic nervous system and neural crest derivatives; also designated HAND2) and eHAND (also designated HAND1, HXT or Thing1) are members of a subclass of basic-helix-loop-helix transcription factors that are involved in cardiac development. dHAND and eHAND are expressed in the heart after cardiac looping, and they participate in left-right cardiac asymmetry. dHAND is expressed predominantly on the right side of the looped heart tube and in the pulmonary ventricle, where it activates transcription of various genes, including Ufd1 (for ubiquitin fusion degradation) and Cdc45. In addition, dHAND is expressed in sympathetic neurons and chromafin cells throughout embryonic and fetal development and mediates neural crest development. eHAND expression is primarily observed on the left side and in the systemic ventricle, suggesting that these proteins are involved in the development of segments of the heart tube, which give rise to specific heart chambers during cardiogenesis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: HAND1 polyclonal antibody detects endogenous levels of HAND1 protein.

Bioworld Molecular Weight: ~ 24 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HAND1 polyclonal antibody at 1:500
Line1:Hela whole cell lysate
Line2:H9C2 whole cell lysate
Line3:

Immunogen: A synthetic peptide corresponding to residues in Human HAND1.

Conjugate: Unconjugated

Modification: Unmodified