HAO1 Polyclonal Antibody-BS67321

HAO1 Polyclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: BS67321-50, BS67321-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9UJM8

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC

Background: Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate.The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine; this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones.Can also catalyze the oxidation of glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate, albeit with much lower catalytic efficiency.Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2.Is not active on L-lactate and 2-hydroxybutanoate.

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of HAO1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of HAO1 expression in mouse liver (A), rat liver (B) whole cell lysates., Immunohistochemical analysis of HAO1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~40 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: GOX1; HAOX1; Hydroxyacid oxidase 1; HAOX1; Glycolate oxidase; GOX

Immunogen: Recombinant full length protein of human HAO1

Conjugate: Unconjugated

Modification: Unmodified