Haptoglobin Polyclonal Antibody-BS90611

Haptoglobin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90611-50, BS90611-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P00738(Human) Q61646(Mouse) P06866(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, ICC/IF

All Applications: WB:1:500-1:2,000
IHC:1:50-1:200
IHC:1:50-1:200

Background: Haptoglobin (Hp) is a blood plasma protein that functions to bind free Hemoglobin that has been released from erythrocytes, thereby inhibiting its oxidative activity. During this process, Haptoglobin sequesters the iron within Hemoglobin, preventing iron-utilizing bacteria from benefitting from hemolysis. This function suggests that Haptoglobin concentrations may increase in response to inflammation. The resulting Haptoglobin-Hemoglobin complex is then removed by the reticulo-endothelial system. Due to cleavage of a common precursor protein during protein synthesis, Haptoglobin consists of two α and two β chains, connected by disulfide bridges. In human, Haptoglobin exists in two allelic forms designated Haptoglogin 1 (Hp1) and Haptoglobin 2 (Hp2), where Hp2 is the result of a partial Hp1 gene duplication. There are three known phenotypes of human Haptoglobin: Hp1-1, Hp2-1 and Hp2-2, which may be associated with diabetes and cardiovascular disease pathology and a susceptibility to Parkinson's and Crohn's disease. Haptoglobin levels are useful in diagnosing hemolytic anemia, the abnormal breakdown of red blood cells. Haptoglobin is expressed in mammalian hepatocytes as well as other tissues such as skin, lung and kidney.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Haptoglobin polyclonal antibody detects endogenous levels of Haptoglobin protein.

Bioworld Molecular Weight: 45/38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Haptoglobin on different cells lysates using anti-Haptoglobin antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2, ICC staining Haptoglobin in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: HP, Binding peptide, BP, Haptoglobin alpha chain, Haptoglobin alpha(1S) beta, Haptoglobin alpha(2FS) beta, Haptoglobin beta chain, Haptoglobin, alpha polypeptide, Haptoglobin, beta polypeptide, HP, HP2 ALPHA2, HP2ALPHA2, HPA1S, HPT, HPT, MGC111141, Zonulin,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified