HAUS1 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS77183-50, BS77183-100 Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: Q96CS2 Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB All Applications: WB,1:500 - 1:2000 Background: HAUS1 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]). Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Unmodification Extra Notes: Western blot analysis of extracts of various cell lines, using HAUS1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. Note: For research use only, not for use in diagnostic procedure. Bioworld Molecular Weight: 32kDa Alternative Name: HAUS1; CCDC5; HEI-C; HEIC; HsT1461 Immunogen: Recombinant fusion protein of human HAUS1(NP_612452.1). Conjugate: Unconjugated Modification: Unmodified