HBG1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90613-50, BS90613-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P69891(Human) P69892(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF, IHC
All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1：50-1：100
Background: Hemoglobin (Hgb) is coupled to four iron-binding, methene-linked tetrapyrrole rings (heme). The α (16p13.3; 5′-ζ-pseudoz-pseudo α2-pseudo α1-α2-α1-?1-3′) and β (11p15.5) globin loci determine the basic hemoglobin structure. The globin portion of hemoglobin consists of two α chains and two β chains arranged in pairs forming a tetramer. Each of the four globin chains covalently associates with a heme group. The bonds between α and β chains are weaker than between similar globin chains, thereby forming a cleavage plane that is important for oxygen binding and release. High affinity for oxygen occurs upon relaxation of the α1-β2 cleavage plane. When the two α1-β2 interfaces are closely bound, hemoglobin has a low affinity for oxygen. Hb A, which contains two α chains plus two β chains, comprises 97% of total circulating hemoglobin. The remaining 3% of total circulating hemoglobin is comprised of Hb A-2, which consists of two α chains plus two δ chains, and fetal hemoglobin (Hb F), which consists of two α chains together with two γ chains.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: HBG1 polyclonal antibody detects endogenous levels of HBG1 protein.
Bioworld Molecular Weight: 16 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of HBG1/2 on human placenta (1) and human brain (2) tissue lysates using anti-HBG1/2 antibody at 1/500 dilution., ICC staining HBG1/2 in D3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: HBG1,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified