Product Description

HCCA2 Polyclonal Antibody

Catalogue Numbers: BS60058-50, BS60058-100

Sizes: 50µl, 100µl

Alternative Name: MOB kinase activator 2; Mob2 homolog; Mps one binder kinase activator-like 2; MOB2; HCCA2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q70IA6

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: HCCA2 (Hepatocellular carcinoma-associated gene 2), also known as MOB2 (Mps one binder kinase activator-like 2), is a 467 amino acid protein that belongs to the MOB1/phocein family. HCCA2 is expressed in lung, spleen, brain, and fetal liver. It is highly expressed in hepatocellular carcinoma. HCCA2 is localized in the perinuclear region of the cytoplasm in liver cancer tissues and colocalizes with MAD2L2 in the nucleus of Hela cells. HCCA2 is chracaterized by two N-glycosylation sites, six N-myristolylation sites, two Src homolgy 3 (SH3), and several phosphorylation motifs which indicate that this protein may play a role in an intracellular signal transduction cascade. HCCA2 binds to and regulates the autophosphorylation of the related human serine/threonine kinase 38 (NDR1) and serine/threonine kinase 38L (NDR2). It has been shown that HCCA2 plays a critical role in cell cycle regulation. Overexpression of the protein during the G0/G1 phase inhibits cell proliferation causing cell cycle arrest.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: HCCA2 polyclonal antibody detects endogenous levels of HCCA2 protein.

Bioworld Molecular Weight: ~ 28 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HCCA2 polyclonal antibody at 1:500 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Rat testis tissue lysate

Immunogen: A synthetic peptide corresponding to residues in Human HCCA2.

Conjugate: Unconjugated

Modification: Unmodified