Product Description

HCFC1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76982-50, BS76982-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P51610

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of various cell lines, using HCFC1 antibody at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 120-260kDa

Alternative Name: HCFC1; CFF; HCF; HCF-1; HCF1; HFC1; MRX3; PPP1R89; VCAF

Immunogen: Recombinant fusion protein of human HCFC1(NP_005325.2).

Conjugate: Unconjugated

Modification: Unmodified