Product Description

HCoV-NL63 Spike S2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78081-50, BS78081-100

Swiss-Prot: Q6Q1S2

Host: Rabbit

Reactivity: HCoV-NL63

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: S1 region attaches the virion to the cell membrane by interacting with host ACE2, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of Human coronavirus Spike Protein , using HCoV-NL63 Spike S2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Bioworld Molecular Weight: 160KDa

Note: For research use only, not for use in diagnostic procedure.

Immunogen: A synthetic peptide of coronavirus Spike S2(YP_003767.1).

Conjugate: Unconjugated

Modification: Unmodified