Product Description

HCoV-OC43 NucleoProtein Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78080-50, BS78080-100

Swiss-Prot: P33469

Host: Rabbit

Reactivity: HCoV-OC43

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of Human coronavirus Nucleocapsid Protein , using HCoV-OC43 Nucleoprotein antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Bioworld Molecular Weight: 50KDa

Note: For research use only, not for use in diagnostic procedure.

Immunogen: A synthetic peptide of coronavirus Nucleoprotein(YP_009555245.1).

Conjugate: Unconjugated

Modification: Unmodified