HCoV-OC43 Spike S1 Polyclonal Antibody-BS80272

HCoV-OC43 Spike S1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80272-50, BS80272-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P36334

Host: Rabbit

Reactivity: HCoV-OC43

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: S1 attaches the virion to the cell membrane by interacting with sialic acid-containing cell receptors, initiating the infection.; [Spike protein S1]: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 110KDa/145KDa

Immunogen: A synthetic peptide of coronavirus Spike S1(YP_009555241.1).

Conjugate: Unconjugated

Modification: Unmodified

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of Human coronavirus Spike Protein, using HCoV-OC43 Spike S1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.