HDAC1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90615-50, BS90615-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q13547(Human) O09106(Mouse) Q4QQW4(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription. HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes. In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity. Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases. Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: HDAC1 polyclonal antibody detects endogenous levels of HDAC1 protein.
Bioworld Molecular Weight: 65 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of HDAC1 on different lysates using anti-HDAC1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: K562, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HDAC1 antibody. Counter stained with hematoxylin.
Alternative Name: HD1, HDAC1, DKFZp686H12203, GON 10, HD1, HDAC 1, HDAC1, HDAC1, Histone deacetylase 1, Reduced potassium dependency yeast homolog like 1, RPD3, RPD3L1,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified