Product Description

HDAC2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90617-50, BS90617-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q92769(Human) P70288(Mouse) Unigene:1797(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation, and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: HDAC2 polyclonal antibody detects endogenous levels of HDAC2 protein.

Bioworld Molecular Weight: 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of HDAC2 on different lysates using anti-HDAC2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: K562, ICC staining HDAC2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: HD2, HDAC2, D10Wsu179e, HD 2, HD2, HDAC 2, Hdac2, HDAC2, Histone deacetylase 2 (HD2), Histone deacetylase 2, OTTHUMP00000017046, OTTHUMP00000227077, OTTHUMP00000227078, RPD3, transcriptional regulator homolog RPD3, YAF1, YY1 associated factor 1, YY1 transcription factor binding protein, Yy1bp,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified