HDAC3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90619-50, BS90619-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O15379(Human) O88895(Mouse) Q6P6W3(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP,
All Applications: WB:1:1,000-1:5,000
ICC:1:50
IHC:1:50-1:200
Background: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional factor Rpd3p, have been identified as histone deacetylases.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: HDAC3 polyclonal antibody detects endogenous levels of HDAC3 protein.
Bioworld Molecular Weight: 49 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of HDAC3 on NIH/3T3 cell lysates using anti-HDAC3 antibody at 1/1,000 dilution., ICC staining HDAC3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: HD3, HDAC3, HD3, HDAC 3, HDAC3, HDAC3, Histone deacetylase 3, RPD3 2, RPD3, RPD3-2, SMAP45,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified