HDAC8 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90624-50, BS90624-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9BY41(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Background: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: HDAC8 polyclonal antibody detects endogenous levels of HDAC8 protein.
Bioworld Molecular Weight: 42 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of HDAC8 on different lysates using anti-HDAC8 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: K562, ICC staining HDAC8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: HD8, HDAC8, CDA07, CDLS5, HD 8, HD8, HDAC 8, Hdac8, HDAC8, HDACL 1, HDACL1, Histone deacetylase 8, Histone deacetylase like 1, MRXS6, RPD 3, RPD3, WTS,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified