HDAC9 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90625-50, BS90625-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9UKV0(Human)
Host: Rabbit
Reactivity: Human, Rat
Applications: WB, ICC/IF, IHC, IP,
All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
IP:1:10-1:50
Background: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is a critical component of transcriptional regulation and the acetylation of nucleosomal histones is a major source of this remodeling. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Several mammalian proteins function as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Conversely, the deacetylation of histones is associated with transcriptional silencing. The histone deacetylases (HDAC) include HDAC1-9. HDAC9 and HDAC9a are two alternatively spliced isoforms of HDAC9. HDAC9a is 132 amino acids shorter than HDAC9, but both isoforms contain the HDAC catalytic domain, remain capable of deacetylase activity and repress myoctye enhancer-binding factor 2-mediated transcription.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: HDAC9 polyclonal antibody detects endogenous levels of HDAC9 protein.
Bioworld Molecular Weight: 140 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of HDAC9 on different lysates using anti-HDAC9 antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562
Lane 2: HepG2
Lane 3: Raji, ICC staining HDAC9 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: HD9, HD7, HD7b, HDAC9, HDAC7, HDAC7B, HDRP, KIAA0744, MITR, HD 7, HD 7B, HD 9, HD7, HD7B, HD9, HDAC 7, HDAC 7B, HDAC 9, HDAC 9B, HDAC 9FL, HDAC, HDAC7, HDAC7B, HDAC9, HDAC9, HDAC9B, HDAC9FL, HDRP, Histone deacetylase 4/5 related protein, Histone deacetylase 7, Histone deacetylase 7B, Histone deacetylase 9, Histone deacetylase 9A, Histone deacetylase related protein, Histone deacetylase-related protein, KIAA0744, MEF2 interacting transcription repressor MITR, MEF2 interacting transcription repressor protein, MEF2-interacting transcription repressor MITR, MITR,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified