HE4 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90626-50, BS90626-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q14508(Human) Q9DAU7(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: HE4 (whey acidic protein (WAP)-type four-disulfide core-2, WFDC2) is a small secretory protein that may influence sperm maturation. HE4 gene expression is high in pulmonary epithelial cells and in some ovarian cancers. HE4 protein has a WAP motif that contains eight cysteines forming four disulfide bonds at the core of the protein. The WAP motif functions as a protease inhibitor in many of the family members that contain them.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: HE4 polyclonal antibody detects endogenous levels of HE4 protein.
Bioworld Molecular Weight: 23 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of HE4 on different lysates using anti-HE4 antibody at 1/1,000 dilution.
Positive control:
Lane 1: SW480
Lane 2: SKOV-3, ICC staining HE4 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: WFDC2, dJ461P17.6, EDDM4, epididymal protein 4, Epididymal secretory protein E4, Epididymis specific whey acidic protein type four disulfide core, HE 4, Major epididymis specific protein E4, Major epididymis-specific protein E4, MGC57529, Putative protease inhibitor WAP5, WAP 5, WAP domain containing protein HE4, WAP domain containing protein HE4 V4, WAP four disulfide core domain 2, WAP four disulfide core domain protein 2, WAP four-disulfide core domain protein 2, WAP5, WFDC 2, WFDC2, WFDC2,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified