Product Description

HEC1 Polyclonal Antibody

Catalogue Numbers: BS5755-50, BS5755-100

Sizes: 50µl, 100µl

Alternative Name: Kinetochore protein NDC80 homolog; Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC; NDC80; HEC1; KNTC2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O14777

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IF

All Applications: WB: 1:500~1:1000
IF: 1:50~1:200

Background: Highly expressed in cancer (Hec1) is a coiled-coil-enriched protein expressed abundantly in the S and M phases of rapidly dividing cells where it localizes to the kinetochores. Hec1 is involved in spindle checkpoint signaling. Hec1 is not expressed in terminal differentiated cells. Hec1 is expressed in tissues with high mitotic rates including testis, spleen and thymus. Hec1 is also found in the late S to M phases of bladder carcinoma cells. In dividing cells, Hec1 is required for the recruitment of Mps1 kinase and MAD1/MAD2 complexes to the kinetochores. The phosphorylation of Hec1 on Serine 165 by Nek2 is essential for faithful chromosome segregation. The binding of retinoblastoma protein to Hec1 also increases the fidelity of chromosomal segregation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: HEC1 polyclonal antibody detects endogenous levels of HEC1 protein.

Bioworld Molecular Weight: ~ 74 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HEC1 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to the 388-433 of Human HEC1.

Conjugate: Unconjugated

Modification: Unmodified