Product Description

HEC1 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS5755-50, BS5755-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Highly expressed in cancer (Hec1) is a coiled-coil-enriched protein expressed abundantly in the S and M phases of rapidly dividing cells where it localizes to the kinetochores. Hec1 is involved in spindle checkpoint signaling. Hec1 is not expressed in terminal differentiated cells. Hec1 is expressed in tissues with high mitotic rates including testis, spleen and thymus. Hec1 is also found in the late S to M phases of bladder carcinoma cells. In dividing cells, Hec1 is required for the recruitment of Mps1 kinase and MAD1/MAD2 complexes to the kinetochores. The phosphorylation of Hec1 on Serine 165 by Nek2 is essential for faithfμl chromosome segregation. The binding of retinoblastoma protein to Hec1 also increases the fidelity of chromosomal segregation.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IF

Alternate Names: Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, HsHec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC, NDC80, HEC1, KNTC2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to the 388-433 of Human HEC1.

Dilution: WB: 1:500~1:1000
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: HEC1 polyclonal antibody detects endogenous levels of HEC1 protein.

Molecular Weight: ~ 74 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O14777

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only