HEC1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90627-50, BS90627-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O14777(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, FC
All Applications: WB:1:500
FC:1:50-1:100
Background: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: HEC1 polyclonal antibody detects endogenous levels of HEC1 protein.
Bioworld Molecular Weight: 74 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of HEC1 on SiHa (1,2) and HL-60 (3,4) cell lysates using anti-HEC1 antibody at 1/500 dilution.
Positive control:
Lane1, 3: Anti-HEC1 antibody
Lane2, 4: Anti-HEC1 antibody, with immunization Recombinant protein, Flow cytometric analysis of MG-63 cells with HEC1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Alternative Name: HsHec1, NDC80, HEC, HEC1, KNTC2, from Entrez Gene ; , HEC, Highly expressed in cancer, Highly expressed in cancer protein, Highly expressed in cancer rich in leucine heptad repeats, HsHec1, hsNDC80, Kinetochore associated 2, Kinetochore associated protein 2, Kinetochore protein Hec1, Kinetochore protein NDC80 homolog, Kinetochore-associated protein 2, KNTC2, ndc80, NDC80 homolog kinetochore complex component, NDC80 kinetochore complex component homolog, NDC80, S. cerevisiae, homolog of, NDC80, Retinoblastoma associated protein HEC, Retinoblastoma-associated protein HEC, TID3,
Immunogen: Recombinant protein within human HEC1 300-500 aa.
Conjugate: Unconjugated
Modification: Unmodified