Hel-N1 (S27) Polyclonal Antibody-BS2299

Hel-N1 (S27) Polyclonal Antibody

Catalogue Numbers: BS2299-50, BS2299-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q12926

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The Elav-like genes encode for a family of RNA-binding proteins. Elav, a Drosophila protein and the first described member, is expressed immediately after neuroblastic differentiation into neurons and is necessary for neuronal differentiation and maintenance. Several mammalian Elav-like proteins, designated HuC, HuD and Hel-N1, are also expressed in postmitotic neurons. An additional mammalian homolog, HuR, which is also designated HuA, is ubiquitously expressed and is also overexpressed in a wide variety of tumors. Characteristically, these homologs all contain three RNA recognition motifs (RRM), and they specifically bind to AU-rich elements (ARE) in the 3'-untranslated region of mRNAs transcripts. ARE sites target mRNA for rapid degradation and thereby regulate the expression levels of genes involved in cell growth and differentiation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Hel-N1 (S27) polyclonal antibody detects endogenous levels of HuB protein.

Extra Notes: Western blot (WB) analysis of Hel-N1 (S27) pAb at 1:500 dilution
Lane1:The Brain tissue lysate of Mouse(40ug)
Lane2:C6 whole cell lysate(40ug)
Lane3:U-87MG whole cell lysate(40ug)
Lane4:PC3 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: ELAV like 4; ELAV like neuronal protein 1; ELAV like protein 2; ELAV like protein 4; ELAVL 2; ELAVL 4; HELN1; Hu antigen B; Hu antigen D; HUB; HUD; Hel-N1; ELAVL2; HEL-N1; HELN1; HUB

Immunogen: Synthetic peptide, corresponding to the N-terminus of Human Hel-N1.

Conjugate: Unconjugated

Modification: Unmodified