Product Description

HER3 (Phospho-Y1222) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS64529-50, BS64529-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Fμll length ErbB-3 is overexpressed in human mammary tumors. The ErbB-3 gene also produces several alternative variants, including a secreted form which negatively regμlates heregμlin stimμlated ErbB activation. ErbB-3 heterodimerizes with Neu and binds heregμlin in order to activate phosphoinositide (PI) 3-kinase. The recruitment and activation of PI 3-kinase occurs via its interaction with phosphorylated YXXM motifs in the carboxy terminus of ErbB-3.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: ERBB3, ERBB3 protein, erbB3 S, ERBB3, Glial growth factor receptor, HER-3, HER3, LCCS2, MDA BF 1, MGC88033, p180 ErbB3, p45 sErbB3, p85 sErbB3, proto-oncogene-like protein c ErbB 3,

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human HER3 around the phosphorylation site of Tyrosine 1222.

Dilution: WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: HER3 (Phospho-Y1222) polyclonal antibody detects endogenous levels of HER3 protein only when phosphorylated at Tyr1222.

Molecular Weight: ~ 220 kDa

Pathway: ErbB HER Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P21860

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only