HFE2 Polyclonal Antibody-BS7548

HFE2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7548-50, BS7548-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q6ZVN8

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: The product of this gene is involved in iron metabolism. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. It could also represent the cellular receptor for hepcidin. Two uORFs in the 5' UTR negatively regulate the expression and activity of the encoded protein. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Defects in this gene are the cause of hemochromatosis type 2A, also called juvenile hemochromatosis (JH). JH is an early-onset autosomal recessive disorder due to severe iron overload resulting in hypogonadotrophic hypogonadism, hepatic fibrosis or cirrhosis and cardiomyopathy, occurring typically before age of 30.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 52kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of extracts of Rat heart, using HFE2 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Alternative Name: HFE2; HFE2A; HJV; JH; RGMC

Immunogen: Recombinant fusion protein of human HFE2(NP_998818.1).

Conjugate: Unconjugated

Modification: Unmodified