Product Description

HIC2 Polyclonal Antibody

Catalogue Numbers: BS60430-50, BS60430-100

Sizes: 50µl, 100µl

Alternative Name: Hypermethylated in cancer 2 protein; Hic-2; HIC1-related gene on chromosome 22 protein
Hic-3; Zinc finger and BTB domain-containing protein 30; HIC2; HRG22; KIAA1020; ZBTB30

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96JB3

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: HIC-2 (hypermethylated in cancer 2) possesses zinc finger motifs that are thought to be important for DNA-binding and also has a BTB/POZ domain at the N-terminus, which is thought to be important for protein-protein binding, as well as for the binding of transcription factors. HIC-2 is also known as Hic-3, HIC1-related gene on chromosome 22 or Zinc finger and BTB domain-containing protein 30, and is a 615 amino acid protein that is expressed as two isoforms produced by alternative splicing. HIC-2 is highly expressed in cerebellum and is localized to the nucleus in cells. HIC-2 contains a short amino acid sequence that is thought to interact with CtBP, a transcriptional repressor. The gene sequence associated with HIC-2 is thought to be a target for miRNAs (microRNAs) which are expressed in many cancers, suggesting that HIC-2 could possess tumor suppressor capabilities.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: HIC2 polyclonal antibody detects endogenous levels of HIC2 protein.

Bioworld Molecular Weight: ~ 72 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HIC2 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: NIH-3T3 cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human HIC2.

Conjugate: Unconjugated

Modification: Unmodified