HIF1AN Monoclonal Antibody-MB12060

HIF1AN Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB12060-50, MB12060-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q9NWT6

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-F, IHC-P, ICC/IF

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 40 kDa; Observed MW: 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunocytochemistry analysis of HIF1AN in HT-1080 using HIF1AN antibody, and DAPI., Western blot analysis of HIF1AN/FIH1 in Hela, A549, HL-60, U2OS, U87-MG lysates using HIF1AN/FIH1 antibody., Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using HIF1AN/FIH1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: FIH 1; FIH1; HIF1AN

Immunogen: A synthetic peptide of human HIF1AN/FIH-1

Conjugate: Unconjugated

Modification: Unmodified