Product Description

HIPK2 Polyclonal Antibody

Catalogue Numbers: BS60320-50, BS60320-100

Sizes: 50µl, 100µl

Alternative Name: Homeodomain-interacting protein kinase 2; hHIPk2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9H2X6

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The Homeodomain-Interacting Protein Kinase (HIPK) family, which includes HIPK1, HIPK2, HIPK3, contains a conserved protein kinase domain as well as a separate domain that interacts with homeoproteins. HIPK2, the most highly characterized family member, is thought to act as a co-repressor of homeodomain transcription factors as HIPK2 has been shown to enhance the DNA binding of the NK-3 homeoprotein in vitro. It is regulated by a posttranslational modification of a ubiquitin-like protein, SUMO-1, via covalent bonding to a lysine residue on HIPK2. This is similar to the binding of SUMO-1 to PML and Sp100. The conjugation of SUMO-1 is thought to direct each of these proteins to nuclear bodies (NBs), which appear to play a role in autoimmunity and viral protection. HIPK2 is the first protein kinase to be directed to nuclear bodies in response to ubiquitin-like modification.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: HIPK2 polyclonal antibody detects endogenous levels of HIPK2 protein.

Bioworld Molecular Weight: ~ 110 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HIPK2 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human HIPK2.

Conjugate: Unconjugated

Modification: Unmodified