Product Description

His-tag (4C2) Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: AP0032M-50, AP0032M-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successfμl relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag.

Category: Primary Antibody

Reactivity: Transfected

Host: Mouse

Applications: WB, IP, IF

Alternate Names: 6 His epitope tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, His tag

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide His-tag.

Dilution: WB:1:5000~10000
IP:1:50~100
IF:1:50~200

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: His-tag (4C2) Monoclonal Antibody detects over-expressed or recombinant proteins containing the 6×His epitope tag.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only