His-Tag Monoclonal Antibody - E-AB-20009

His-Tag Monoclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-20009-60, E-AB-20009-120, E-AB-20009-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: His-Tag

Target Synonym: 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag; Polyhistidine Tag

Research Areas: Tags and Cell Markers

Conjugation: Unconjugated

Host: Mouse

Application: WB, IF, IP

Isotype: IgG

Clonality: Monoclonal

Clone No.: Clone:5I8

Background: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

Concentration: 1 mg/mL

Immunogen: Synthetic Peptide

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Purification Method: Protein A purification

Dilution: WB 1:5000-1:10000, IF 1:500-1:2000, IP 1:100-1:300

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only